顺铂诱导肾性贫血大鼠模型的建立

Establishment of rat model of DDP-induced renal anemia

摘要:
目的建立顺铂(DDP)诱发肾性贫血的大鼠模型。
方法给大鼠尾静脉注射8 mg/kg DDP, 采用胎肝细胞3H-TdR渗入法检测大鼠血清红细胞生成素(EPO)的含量, 并观察大鼠肾组织在光镜及电镜下形态的改变及重组人红细胞生成素(rhEPO)对DDP诱发贫血的影响。
结果 DDP可引起大鼠红细胞(RBC)、血红蛋白(Hb), 网织红细胞(Ret)及血清EPO水平明显降低, 血清尿素氮(BUN)水平显著升高。病理切片也证实DDP可引起大鼠明显的肾损伤。rhEPO能明显升高大鼠Hb的水平, 并有明显的量效关系。
结论 8 mg/kg DDP可诱导大鼠肾性贫血模型。

Abstract:
Objective To establish the rat model of cis-diamine-dichloro-platinum(DDP)-induced renal anemia.
Methods The rats were intravenously injected with DDP(8mg/kg), and serum EPO levels were measured by 3H-TDR incorporation in fetal mouse hepato cytes.Moreover, the changes of renal tissue were studied by light microscope and electron microscope.
Results The number of red blood cell, hemoglobin concentration, reticulocytes and serumer ythropoietin(EPO) were greatly decreased, serum urea nitrogen elevated significantly, pathological findings revealed that DDP might significantly cause renal damage in rats.
Conclusion Rat model of renal anemia could be induced by DDP.