HBV、HIV及混合感染者T淋巴细胞水平分析

陈显兵; 管小琴; 吴小川; 杨练; 肖明

doi:10.11847/zgggws2006-22-05-07

HBV、HIV及混合感染者T淋巴细胞水平分析

Analysis on T lymphocyte level in patients infected with HBV/HIV and HIV/HBV co-infected

摘要

摘要:
目的定量分析乙型肝炎病毒(HBV)、人类免疫缺陷病毒(HIV)及混合感染者CD3⁺、CD4⁺、CD8⁺T淋巴细胞水平。
方法采用流式细胞技术(FCM)对31例HBV感染者、46例HIV感染者、14例HBV/HIV混合感染者及18例正常对照者分别检测CD3⁺、CD4⁺、CD8⁺淋巴细胞绝对计数和CD4⁺/CD8⁺比值。
结果 HIV/HBV混合感染组, HIV感染组、HBV感染组及健康对照组各项指标间差异有统计学意义(F=50.67, F=46.06, F=16.13, F=92.51, P < 0.01);CD3⁺细胞计数呈现为HIV/HBV组 < HIV组 < HBV组 < 正常组(P < 0.01);CD4⁺细胞计数表现为HIV/HBV组 < HIV组 < HBV组 < 正常组(P < 0.01);CD8⁺细胞计数表现为HIV组 > HBV组 > 正常组(P < 0.01)。CD4⁺/CD8⁺比值降低, HIV/HBV组、HIV组 < HBV组 < 正常组(P < 0.01)。
结论 CD4⁺细胞绝对计数和CD4⁺/CD8⁺比值的检测在评价HIV感染者的免疫状况、进展和预后判断等方面具有重要作用。HIV/HBV混合感染有可能加重机体免疫功能的损害。

Abstract:
Objective To quantitatively and qualitatively analyse the CD3⁺ /CD4⁺ /CD8⁺ T lymphocy te level of patients infected with HBV or HIV, and polyinfected with HBV and HIV.
Methods The flow cytometry was used to measure CD3⁺ /CD4⁺ /CD8⁺ and CD4⁺ /CD8⁺ ratio of 31 patients infected with HBV, 46 patients infected with HIV, 14 patients polyinfected with HBV and HIV and 18 normal controls.
Results All markers of HIV/HBV polyinfection group, HIV infection group, HBV infection group and normal control group had statistics differ entiations with each other(F=50.67, F= 46.06, F=16.13, F=92.51, P < 0.01);Quantity of CD3⁺ cells showed that HIV/HBV group < HIV group < HBV group < normal control(P < 0.01), quantity of CD4⁺ cells was HIV/HBV group < HIV group < HBV group < normal control(P < 0.01), while quantity of CD8⁺ cells was HIV group > HBV group > normal control(P < 0.01).D4+/CD8⁺ ratio is invert, HIV/HBV, HIV group < HBV group < normal control(P < 0.01).
Conclusion the detection of absolute quantity of CD4⁺ cells and CD4⁺/CD8⁺ ratio maybe important in evaluating immunecondition, progression and prognosis of patients infected with HIV.moveover, polyinfected with HBV and HIV may hurt the patients immune fundtion more serious.

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