微囊藻毒素和黄曲霉毒素及伏马菌素联合毒性

熊丽林; 唐萌; 王加生; 曾垂焕; 夏婷; 王晓娜

doi:10.11847/zgggws2006-22-05-25

微囊藻毒素和黄曲霉毒素及伏马菌素联合毒性

Combinative toxicity of Microcystin, Aflatoxin and Fumonisin

摘要

摘要:
目的研究微囊藻毒素、黄曲霉毒素、伏马菌素对Hep G2细胞的半数抑制浓度(IC₅₀), 探讨3种毒素的联合毒性。
方法选用3种毒素各自最具代表性的异构体微囊藻毒素LR、黄曲霉毒素B₁和伏马菌素B₁, 用水溶性四氮唑(WST-1)比色法测定3种物质对Hep G2细胞存活率的影响, 计算IC₅₀, 并根据等效曲面联合毒性分析模型判断联合作用类型。
结果染毒24 h, 微囊藻毒素LR对Hep G2细胞的IC₅₀ > 100μmol/L; 黄曲霉毒素B₁、伏马菌素B₁及3毒素混合物的IC₅₀分别为1.0, 399.2和172.0μmol/L。根据等效曲面法求得的相互作用指数α为0.95。
结论微囊藻毒素LR、黄曲霉毒素B₁和伏马菌素B₁对Hep G2的联合毒作用类型为相加作用。

Abstract:
Objective To study 50% inhibition concentration(IC₅₀)of Microcystin, Aflatoxin and Fumonisin and the combinative toxic effect of the three biotoxins in Hep G2 cell line.
Methods Cell viability and 50% inhibition concentration (IC₅₀)of Microcystin-L R(MCLR), Aflatoxin B₁(AFB₁), Fumonisin B₁(FB₁)and the mixture were measured by tetrazolium salt(WST-1)assay with Hep G2 cell line.Combinative toxicity of the three biotoxins was evaluated according to response surface analysis.
Results Treatment of MCLR for 24, the IC₅₀ was more than 100μmol/L.IC₅₀ of Aflatoxin B₁, Fumonisin B₁ and the mixture of the three biotoxins were 1.0, 399.2 and 172.0μmol/L respectively.The interaction index according to response surface analysis was 0195.
Conclusion Combinative toxicity of the mixture falls into the category of additive effect.

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