家蝇幼虫抗菌蛋白的诱导及抗菌特性

刘晖; 万启惠; 贺莉芳; 张曦

doi:10.11847/zgggws2006-22-05-37

家蝇幼虫抗菌蛋白的诱导及抗菌特性

Study on inducement and antibacterial characterization of antibacterial proteins in Musca domestica larvae

摘要

摘要:
目的用不同诱导源诱导家蝇幼虫, 观察抗菌蛋白产生的规律及其抑菌特性, 为进一步研究家蝇幼虫抗菌蛋白的产生及应用提供实验依据。
方法用4种诱导源诱导后不同时间的组织匀浆提取抗菌蛋白进行抑菌实验, 观察不同诱导源诱导后抗菌蛋白产生的高峰时间及各诱导组抗菌蛋白的抑菌特性。
结果各诱导组抗菌蛋白的抑菌活性明显高于对照组, 各诱导组抗菌蛋白的产生在诱导后36~48 h达高峰; 各诱导组产生的抗菌蛋白均能抑制几种革兰阴性菌、一种革兰阳性菌和耐药菌株, 但对不同细菌的抑菌活性有差别。
结论不同诱导源诱导家蝇幼虫后抗菌蛋白产生的高峰时间不同、产生的抗菌蛋白抗菌谱广。

Abstract:
Objective To observe the rule of antibacterial activity and the antibacterial characterization of the ant ibacterial proteins in the Musca domestica larvae by different inducements.To provide the experiment proof for the antibacterial proteins producing and application of the Musca domestica larvae.
Methods the Musca domestica larvae were induced by four factors, antibacterial prot eins were separated from the tissue homogenate at the different time, detected their antibacterial activity to observe the peak of the antibacterial proteins.Several different bacteria were used as detecting index to detect the antibacterial character ization of antibacterial proteins which induced by four inducements.
Results the antibacterial proteins o f antibacterial activity of four induced groups were better than control group, and the peak of antibacter ial activity appeared at 36~48 h after induced by four inducements'these ant ibacterial proteins could inhabit many bacteria(including some Gram-epositive, Grame-negative bacteria and rifampin resistant E.coli), but ant ibacterial activity were different.
Conclusion the peak of antibacter ial proteins in the Musca domestica larvae by different inducements appeared at different time, and could inhibit diversiform bacteria.

HTML全文

参考文献(7)

施引文献

资源附件(0)