双酚A对雄性小鼠生殖细胞凋亡作用
Effect of bisphenol-A on apoptosis of male mice reproductive cells
-
摘要:目的 研究双酚A(BPA)对雄性小鼠生殖细胞的凋亡作用。方法 选择雄性昆明种小鼠, 连续5 d双酚A腹腔注射, 剂量为0, 250, 500, 1 000μmol/kg。分别于首次染毒后的第7和14 d每组随机处死7只小鼠。用流式细胞仪测定其睾丸细胞周期, 称睾丸重量及计算小鼠脏器系数, 测定睾丸组织中一氧化氮(NO)含量和一氧化氮合酶(NOS)的活性。结果 不同剂量组睾丸细胞各时相的细胞百分数发生变化, G0/G1和S期细胞百分数明显减少, G2+M期时相的细胞百分数逐渐增加, Ap峰所占比例增加, 染毒组睾丸脏器系数低于对照组, NO含量和NOS的活性均高于对照组, 染毒组与对照组比较差异有统计学意义。结论 双酚A可使雄性小鼠睾丸细胞的DNA合成受抑制, 使睾丸细胞出现G2期阻滞, 有丝分裂延迟, 使进入M期的细胞百分数减少, 表明双酚A对小鼠生殖细胞有凋亡作用。同时, NO、NOS指标的升高也反映了小鼠生殖细胞受损与脂质过氧化有关。Abstract:Objective To study the effects of bisphenol-A(BPA)on apoptosis of male mice reproductive cells.Methods Male mice in experiment groups were exposed to BPA by using the intraperitoneal injection for 5d at the dose of 0, 250, 500, 1000μmol/kg respectively.Seven mice were randomly sacrificed in each group on the 77th and 14th day after first exposure to BPA.The flow cytometry was used to study the effects of BPA on testicle cells of male mice.The testicular viscera coefficient were measured and the contents of nitric oxide(NO)and nitric oxide synthase(NOS)were tested.Results The different dose of BPA could make the testicle cell cycle change.The cells in G0/G1, Sphases decreased, the accounts of cells in G2/M increased and Ap part increased with the increase of BPA.The testicular viscera coefficient decreased in the experimental groups compared with that in the blank group.The contents of NO and NOS in the testicles of mice were higher than that in the blank group.There were significant differences between experimental groups and blank control.Conclusion BPA can inhibit DNA synthesis of testicle cells, cause the G2 block and delay the mitosis of testice cells, and the cells in Mphase decreased with the increase of BPA.It shows that BPA may affect the reproductive cells on apoptosis of male mice.The content of NO and NOS increased to show that the damaged reproductive cells are related with lipid peroxide.
下载: