邻苯二甲酸(2-乙基已基)酯对小鼠脏器损伤作用
Effects of Di-2-ethylhexylphalate on viscera in mice
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摘要:目的 了解邻苯二甲酸酯(2-乙基已基)(DEHP)对小鼠各组织器官造成的损伤。方法 将昆明种小鼠进行60 d喂养实验后随机分为5组, DEHP浓度为250, 500, 1 000, 2 000μg/ml等4个剂量组, 称量体重和各脏器湿重并计算脏器系数。结果 较高剂量组小鼠在染毒10 d后, 体重停止增长且有体重减轻的现象(P < 0.05, P < 0.01), 雌鼠接触DEHP 60 d之后, 各染毒剂量组小鼠其心脏/体、肺/体系数与对照组相比都增加(P < 0.05, P < 0.01);肝脏/体、肾脏/体系数除250μg/ml剂量组外与对照组相比均有增长的趋势(P < 0.05, P < 0.01)。雄鼠接触DEHP 60 d后, 各染毒剂量组小鼠其心脏/体、肝脏/体、肺/体系数与对照组相比均增加(P < 0.05, P < 0.01);而睾丸/体系数则显著减少(P < 0.05, P < 0.05)。结论 DEHP染毒60 d后对小鼠的脏器造成一定程度的损伤。Abstract:Objective To find out the the effect on Kunming mice viscera made by di-2-ethylhexylphalate(DEHP).Methods Kunming mice viscera were divided into 5 groups in randomization after 60 days feed and the concentration groups of DEHP were 250, 500, 1 000, 2 000 g/ml respectively.To quantify the avoir dupois and wet weight of apieceviscera in mice, then count the coefficient of viscera.Results there was cease and lose weight in the higher dosage group of mice after exposure 10 days(P < 0.05, P < 0.01).the coeffcient of heart/body and lung/body increased in female compared with the control exposure to DEHP after 60 days(P < 0.05, P < 0.01);the co-efficient of liver/body in all groups except 250 g/ml concentration incr eased compared with the control(P < 0.05, P < 0.01).the co-efficient of spermary/body in male groups reduced significantly(P < 0.05, P < 0.01)compared with the control exposure to DEHP after 60 day, but the results in other groups were reversed(P < 0.05, P < 0.01).Conclusion the viscer a of mice were damaged after DEHP-poisoned 60 days.
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