慢性乙肝患者HBVx-DNA表达与mIL-2R相关性

王健; 江水清; 项桂菊

doi:10.11847/zgggws2006-22-10-23

慢性乙肝患者HBVx-DNA表达与mIL-2R相关性

Relationship between expression of HBVx-DNA and mIL-2R in peripheral blood of the patients with chronic hepatitis B

摘要

摘要:
目的探讨慢性乙肝、原发性肝癌患者乙肝病毒x基因(HBVx-DNA)表达水平与膜白细胞介素-2受体(mIL-2R)的相关性。
方法采用聚合酶链反应检测慢性、原发性肝癌乙肝患者血清中HBVx-DNA, 采用生物素-链霉亲和素(BSA)法检测患者外周血单个核细胞(PBMC)用植物血凝素(PHA)诱导前后mIL-2R表达水平。
结果慢性乙肝、原发性肝癌患者HBVx-DNA阳性率分别为11.9%(16/135)和40.9%(9/22), 2者相比差异有统计学意义(P < 0.01)。其中慢性乙肝轻度、中度、重度、肝炎后肝硬化患者血清HBVx-DNA阳性率分别为0, 20.8%(5/24), 17.9%(5/28), 17.1%(6/35), 差异有统计学意义(P < 0.01)。HBeAg(+)组HBVx-DNA阳性率为20.4%(21/103), 与HBeAg(-)组相比, 差异有统计学意义(P < 0.05)。HBVx-DNA(+)组和HBVx-DNA(-)组PHA诱导前后mIL-2R表达水平分别为(2.53±1.08)%, (26.47±4.26)%和(3.34±1.23)%, (29.25±4.71)%, 2者相比差异均有统计学意义(P < 0.01)。肝活组织检查, 原发性肝癌患者HBVx-DNA阳性率达50%(3/6), 明显高于慢性乙肝和肝炎后肝硬化患者。
结论慢性乙肝患者体内有HBVx-DNA存在, 以中度、重度及肝炎后肝硬化患者较多见。原发性肝癌患者体内HBVx-DNA表达水平均最高, 其与宿主细胞整合是导致原发性肝癌发生的重要原因。HBVx-DNA(+)患者T细胞活化功能进一步降低, 可能是诱导原发性肝癌发生的另一重要原因。HBVx-DNA检测对原发性肝癌早期发现和辅助诊断具有重要意义。

Abstract:
Objective To study the relationship between the expression of HBVx-DNA in serum and the level of membrane interleukin-2 receptor(mIL-2R)on surface of peripheral blood mononuclear cells(PBMC)of the patients with chronic hepatitis B and pr imary hepatoma.
Methods The HBVx-DNA in serum and the level of mIL-2R before and after inducement of PHA were detected by PCR and biotin-streptav idin(BSA), respectively.
Results The total positive rates of HBVx-DNA in serum of the patients with chronic hepatitis B and primary hepatoma were 11.9%(16/135), 40.9%(9/22), respectively.There was high significantly difference in two groups(P < 0.01).The positive rates of HBVx-DNA in serum of the patients with chronic hepatitis(light), chronic hepatitis(moderate), chronic hepatitis(severe), cirrhosis were 0%, 20.8%(5/24), 17.9%(5/28), 17.1%(6/35), respectively.There was high significantly difference among these groups (P < 0.01).The total positive rates of HBVx-DNA in serum of group with HBeAg(+)was 2014%(21/103).There was higher significantly difference in group with HBeAg(+)than that in group with HBeAg(-)(P < 0.05).The levels of mIL -2R before and after inducement of PHA on surface of PBMC of group with HBV x-DNA(+)and HBVx-DNA(-) were(2.53±1.08)%, (26.47±4.26)%and(3.34±1.23)%, (29.25±4.71)%, respectively.There was higher significantly difference in the two groups(P < 0.01).The results of liver biopsy showed that the highest level of HBVx-DNA in tissue of hepatoma.
Conclusion HBVx-DNA can be existent in the patients with chronic hepatitis B and is more common in chronic hepatitis B(moderate, severe)and cirrhosis.The positive rate of HBVx-DNA in serum of the patients with primary hepatoma was 50%(3/6)and was hig her than that of patients with chronic hepatitis B(moderate, severe)and cirr hosis.The main reason of primary hepatoma is associated with HBVx-DNA integr ated with chromosome of host cells.The other important reason is that the lowactive T cells is more common in peripheral blood of the patients with HBVx-DNA(+).It is high significant to detect the level of HBVx-DNA in serum and PBMC forearly discovery and assistant diagnosis of primary hepatoma.

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