肠道常见病原菌检测基因芯片的制备与评价

Construction and evaluation of DNA microarray for common intestinal pathogenic bacteria detection

摘要:
目的建立并评价快速准确地检测肠道致病菌的基因芯片检测方法。
方法在基因芯片制备基础上, 通过对靶基因的扩增、杂交和对杂交结果的分析, 对常见肠道致病菌的检测和鉴定效果进行评价。
结果应用包含38种特异性探针的基因芯片, 对涉及沙门菌、志贺菌、霍乱弧菌、致泻性大肠埃希菌、空肠弯曲菌、小肠结肠炎耶尔森菌、副溶血弧菌和单核细胞李斯特菌等8个菌属的16株致病菌进行检测, 均得到特异性杂交图谱。
结论制备的基因芯片能够同时检测多种重要的肠道致病菌, 为肠道致病菌感染的快速诊断提供了准确、快速、灵敏的方法。

Abstract:
Objective To detect the intestinal pathogenic bacteria quickly and accurately.
Methods To detect and identify common intestinal pathogenic bacteria target genes with prepared DNA microarray.
Results 16 strains of bacteria in pure culture belong to 8 genera(salmonella, shigella, escherichia coli, vibrio cholera, vibrioparahaemolyticus, campylobacter jejuni, yersinia enterocolitica, and listeria)were successfully discriminated under comparatively same condition and a series of specific hybridiztion maps corresponding to each kinds of bacteria are obtained.
Conclusion Common intestinal pathogenic bacteria can be detected and identified by DNA microarray.It provides a more efficient method for intestinal pathogenic bacteria detections.