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熊习昆, 杨颖, 谭小华, 郑穗生, 谢晓萍, 李庆. 3T3中性红试验在化妆品光毒检测中的应用[J]. 中国公共卫生, 2006, 22(10): 1207-1209. DOI: 10.11847/zgggws2006-22-10-32
引用本文: 熊习昆, 杨颖, 谭小华, 郑穗生, 谢晓萍, 李庆. 3T3中性红试验在化妆品光毒检测中的应用[J]. 中国公共卫生, 2006, 22(10): 1207-1209. DOI: 10.11847/zgggws2006-22-10-32
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XIONG Xikun, YANG Ying, TAN Xiaohua, . Application of 3T3 neutral red uptake to phototoxicity assay in cosmetic products[J]. Chinese Journal of Public Health, 2006, 22(10): 1207-1209. DOI: 10.11847/zgggws2006-22-10-32
Citation: XIONG Xikun, YANG Ying, TAN Xiaohua, . Application of 3T3 neutral red uptake to phototoxicity assay in cosmetic products[J]. Chinese Journal of Public Health, 2006, 22(10): 1207-1209. DOI: 10.11847/zgggws2006-22-10-32
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3T3中性红试验在化妆品光毒检测中的应用

Application of 3T3 neutral red uptake to phototoxicity assay in cosmetic products

    摘要
  • 摘要:
      目的   研究3T3成纤维细胞中性红摄取试验(3T3 NRU PT)在化妆品安全性评价中取代动物光毒性试验的可行性。
      方法   选用20种国产特殊用途化妆品, 作用丁3T3细胞后, 暴露或不暴露于紫外线下, 测量540 nm处经化学物质和紫外线照射联合作用细胞的吸光度, 分析其平均光效应强度(MPE)、光刺激因子(PIF), 细胞存活率, 以判断待测物质是否具有光毒性, 并与整体动物皮肤光毒性结果进行比较。
      结果   3T3 NRU PT试验测得20种化妆品的PIF值均 < 5, MPE值均 < 0.1, 按该试验阈值分类方法, 均呈光毒性阴性; 动物试验结果表明, 未出现皮肤反应≥2分的动物, 结果也均为光毒性阴性。3T3 NRU PT试验与动物光毒性结果经校正χ2检验比较, 差异无统计学意义, 其灵敏度、特异度、阳性预测价值、阴性预测价值均为1。
      结论   3T3 NRU PT试验和豚鼠皮肤光毒性试验的结果一致, 该方法可取代化妆品终产品的动物光毒性试验。

     

    Abstract:
      Objective   To evaluate the 3T3 Mouse Fibroblast neutral red uptake phototoxicity test(3T3 NRU-PT) method detecting potential phototoxicity of cosmetic products.
      Methods   20 kinds of function cosmetics were detected with the 3T3 NRU-PT(in vitro)and Guinea pig skin phototoxicity test(in vivo), The potential phototoxicity of cosmetics was evaluated in a prediction model in which either the Photo irritation Factor(PIF)or the Mean Photo Effect(MPE)were determined by PHOTO32, using ROC analysis to compare the coherence and sensitive of the method.
      Results   The skin phototoxicity test showed that for the 20 kinds of function cosmetics, all animals responses value(ery thema and edema) < 2.For 3T3 NRU PT.all PIF values < 5, MPE < 0.1, That means whetherin vitro or in vivo, the phototoxicity responses were all negative.The sensitivity(or true positive rate), specificity, positive and negative predictive value, Youden index, Kappa indexall is Ⅰ.The data between in vitro and in vivo was consistent.
      Conclusion   The 3T3 NRUPT testhas the same good internal consistency and reappearance as animal test.It may be a good alternative method for assessing the potential phototoxicity of cosmetic products in future.

     

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