DHPO生殖毒性的毒理学评价
Primary study about reproductive toxicology of DHPO in mice
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摘要:目的 研究1, 2-二甲基-3-羟基-4-吡啶酮(DHPO)的急性毒性及不同剂量、不同时间的生殖毒性。方法 以昆明种近交小鼠为实验对象, 首先进行半数致死量(LD50)实验, 然后对DHPO低(1/8 LD50)、中(1/4 LD50)、高(1/2 LD50)剂量组染毒小鼠进行精子畸变率观察。结果 DHPO染毒小鼠经口LD50为562.34 mg/kg。精子畸变实验显示, 1/8 LD50组、1/4 LD50组与阴性对照组比较, 在第14, 28, 35, 42, 70 d观察, 差异均无统计学意义(P > 0.05);1/2LD50组与阴性对照组比较, 在第14, 28, 35 d观察, 差异均有统计学意义(P < 0.05), 而在第42, 70 d观察, 差异无统计学意义(P > 0.05)。结论 DHPO的低(1/8 LD50)、中(1/4 LD50)剂量未显示有生殖毒性, 高剂量(1/2 LD50)具有一定生殖毒性, 但可在42 d后恢复。Abstract:Objective To evaluate the acute toxicity and reproductive toxicology of deferiprone(DHPO)under various doses and during different periods.Methods Median lethal dose(LD50)test was per for med, then Kun Ming mice were administrated different doses of DHPO to cary out the sperm malformation test under low(1/8 LD50), mid(1/4 LD50), high(1/2 LD50)doses.Results LD50 of DHPO was 562.34 mg/kg by gavage on mice.There were no significant differences in 1/8 LD50 group and 1/4 LD50 group in compar ison with negative control group in obser vations conducted din the 14th, 28th, 35th, 42nd, and 70th day(P > 0.05);While significant differences appeared between 1/2 LD50 group and in negative control group in observations conducted in the 14th, 28th and 35th day(P < 0.05);There were no significant differences between 1/2 L D50 group and negative control group in observations conducted in the 42nd day and 70th day(P > 0.05).Conclusion There is no evidence to indicate that 1/8 LD50, 1/4 LD50 dose have reproductive toxicology(P > 0.05).High dose of DHPO(1/2 LD50) has some degrees of reproductive toxicology, but can recover within 42 days.
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