慢性铅暴露对小鼠CaMKII蛋白表达的影响

文涛; 孙黎光; 刘阳; 赵晓光; 宗志宏; 高明奇; 刘素媛

doi:10.11847/zgggws2006-22-10-34

慢性铅暴露对小鼠CaMKII蛋白表达的影响

Effect of chronic lead exposure on protein expression of CaMKII in mice

摘要

摘要:
目的观察和探讨铅对小鼠海马钙/钙调素依赖性蛋白激酶II(CaMKII)蛋白表达的影响。
方法小鼠出生第1 d起, 染铅组母鼠开始通过饮水饲以不同浓度醋酸铅2.4, 4.8, 9.6 mmol/L。幼鼠出生后, 先通过哺乳接触铅, 断乳后则自行饮用与母鼠饮用浓度相同的含铅水。分别在14, 28, 42, 56 d处死小鼠, 用蛋白免疫印记法观察各组小鼠海马区CaMKII蛋白表达状况。
结果慢性铅暴露对小鼠脑海马CaMK II蛋白表达总体上呈现下降趋势, 2.4 mmol/L染铅组中, 14 d CaMKII蛋白表达呈现上升趋势, 与相应天数对照组相比差异无统计学意义; 4.8, 9.6 mmol/L染铅组中, CaMKII蛋白表达与相应期对照组相比差异有统计学意义(P < 0.05)。
结论铅扰乱CaMKII蛋白正常表达。

Abstract:
Objective To observe the effect of chroniclead contaminant on protein expression of Ca²⁺/calmodulin dependent protein kinase II(CaMK II)in hippocampus of mice.
Methods Chronic acetic lead contaminant was applied on mice(2.4, 4.8, 9.6 mmol/L), and pups were killed at 14, 28, 42, 56 d respectively.Western blotting was used to observe protein expression of CaMKII in hippocampus of mice.
Results Compared with control group, CaMKII protein expression of chronic lead exposure mice in the hippocampus was downtrend.2.4 mmol/L lead exposure could make the protein expression at 14 days higher than that of control(P > 0.01), but at follow days lower than that of control.As to 4.8 mmol/L and 9.6 mmol/L concentration, the protein expression was always lower than that of control(P < 0.05).
Conclusion Chronic lead exposure can disturb the normal change of CaMKII protein expression in mice.

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