番茄红素对人胃癌细胞生长的抑制作用

李垚; 付晶; 潘宏志

doi:10.11847/zgggws2006-22-10-41

摘要:

目的通过体外试验研究番茄红素对人胃癌(SGC)-7901细胞生长的作用。

方法将番茄红素作用于人胃癌SGC-7901细胞, 四甲基偶氮噻唑蓝(MTT)法测定半数抑制浓度(IC₅₀), 细胞计数法和集落形成试验检测细胞生长抑制率, ³H胸腺嘧啶核苷(³H-TdR)掺入试验测定对细胞DNA合成的影响, 倒置显微镜下观察人SGC-7901的形态变化。

结果番茄红素作用于人SGC-7901细胞后, 细胞生长明显受到抑制, 24, 48, 72, 96, 120, 148和172 h的IC₅₀分别为1.0×10^-4, 9.2×10^-5, 2.6×10^-5, 4.8×10^-5, 3.5×10^-5, 4.3×10^-5和3.1×10^-5mol/L。0.3×10^-4, 0.5×10^-4, 10^-4mol/L的番茄红素对人SGC-7901 7 d的抑制率分别为64.75%, 79.02%, 87.13%, SGC-7901的集落形成率分别为(30.2±1.9)%, (22.0±2.1)%, (16.9±2.0)%。番茄红素作用于人SGC-7901 48 h的³H-TdR掺入量分别为(3757.06±114.27), (3148.24±190.23), (2408.32±155.83)cpm, 显著低于阴性对照组的(5470.25±217.16)cpm(P < 0.01)。不同剂量的番茄红素处理后的细胞变圆, 变小, 脱落, 排列稀疏, 部分细胞内出现大小不等的空泡。

结论番茄红素在体外能明显抑制人SGC-7901细胞增殖, 且存在剂量-效应关系。

Abstract:

Objective To investigate the effect of lycopene on growth of human gastric carcinoma cells in vitro .

Methods The human gastric carcinoma SGC-7901 cells were treated with lycopene.IC₅₀ of lycopene on SGC-7901 cells calculated by 3-(4, 5-dimethylthiazole-2-yl)-2, 5 diphenyl tetrazoliumbromide(MTT)assay.The rate of growth suppression on SGC-7901 cells was evaluated by cell growth curve and clone formation.The effect of lycopene on DNA synthesis of human gastric carcinoma SGC-7901 cells was evaluated by ³H-thymidine(³H-TdR)incorporation.Cell morphology was observed by inverted microcope.

Results Lycopene had significantly inhibitory effect on growth of human gastric carcinoma SGC-7901 cells, IC₅₀ of 24, 48, 72, 96, 120, 148, 172h were 1.0×10^-4, 9.2×10^-5, 2.6×10^-5, 4.8×10^-5, 315×10^-5, 413×10^-5 and 3.1×10^-5 mol/L, respectively.Treated with 0.3×10^-4, 015×10^-4、10^-4 mol/L lycopene for 172 h, the rate of cells growth suppression was 64.75%, 79.02%, 87.13%, respectively.Treated with 0.3×10^-4, 0.5×10^-4, 10^-4 mol/L lycopene for 24 h the rate of cloning formation of SGC-7901 cells was(30.2±1.9)%, (22.0±2.1)%, (16.9±2.0)%, respectively.Treated with 0.3×10^-4, 0.5×10^-4, 10^-4 mol/L lycopene for 48 h, ³H-TdR in corporation of human gastric carcinoma SGC-7901 cells were(3757.06±114.27), (3148.24±190.23), (2408.32±155.83)cpm, respectively, which showed significantly decreased(P < 0.01), as compared with that in negative control group which were(5470.25±217.16) cpm; light absorption(value A)of human gastric carcinoma SGC-7901 cells also showed significant decrease(P < 0.01), as compared with that in negative control group.The morphological changes of human gastric carcinoma SGC-7901 cells were observed after treated with 0.3×10^-4, 0.5×10^-4, 10^-4 mol/L lycopene for 24 h, such as rounder, smaller, desquamation, sparse arrangement, vacuole of different size and etc.

Conclusion Lycopene can significantly inhibit proliferation of human gastric carcinoma SGC-7901 cells in dose-response manner in vitro .

番茄红素对人胃癌细胞生长的抑制作用

Effect of lycopene on growth of gastric carcinoma cells