谷胱甘肽与α-硫辛酸对锰致大鼠神经元凋亡影响

刘莉; 徐兆发; 贾克; 徐斌; 杨敬华; 贺安宁

doi:10.11847/zgggws2006-22-10-51

谷胱甘肽与α-硫辛酸对锰致大鼠神经元凋亡影响

Effects of glutathione and alpha-lipoic acid on striatum neuron apoptosis induced by manganese in rats

摘要

摘要:
目的研究谷胱甘肽(GSH)和α-硫辛酸(LA)对锰致大鼠脑组织氧化损伤及纹状体神经元凋亡的影响。
方法取Wistar大鼠32只, 随机分为4组。第1组为对照组, 第2组为单纯染锰组, 第3、4组为干预组。第1组腹腔注射生理盐水, 第2~4组腹腔注射150μmol/(kg·bw)MgCl₂, 2 h后, 第1组和第2组皮下注射生理盐水, 第3组皮下注射35μmol/(kg·bw)LA, 第4组皮下注射1 mmol/(kg·bw)GSH。每周5次, 隔日干预, 共计4周。检测脑组织谷胱甘肽(GSH)、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性; 采用原位末端标记DNA片段(TUNEL)法检测纹状体部位神经元凋亡。
结果单纯染锰组大鼠脑组织MDA含量较对照组升高, GSH和LA干预组MDA较单纯染锰组降低。单纯染锰组神经元凋亡率高于对照组、GSH和LA干预组。
结论 GSH和LA对锰致大鼠脑组织氧化损伤及纹状体神经元的凋亡具有一定的拮抗作用。

Abstract:
Objective To study the effects of g lutathione(GSH)and alpha-lipoic acid(LA)on rats brain oxidative damage and striatum neuron apo ptosis induced by manganese.
Methods 32 Wistar rats were divided into control, manganese chlor ide (MnCl₂), GSH and LA group by weight at random.Control group was injected 0.9% sodium chloride intraperitoneally.Other groups were injected 150 μmol/(kg·bw)MnCl₂ intraper itoneally.2 hours later, control and MnCl₂ group were injected 0.9% sodium chloride subcutaneously, while GSH and LA group were injected 35 μmol/(kg·bw)LA and 1 mmol/(kg·bw) GSH subcutaneously respectively.All groups were administrated 5 times per week, and inter vened once every ot her day.The experimental period was 4 weeks.Then levels of GSH, malony dialdehyde(MDA)and activities of glutathione perox idase(GSH-Px)and superoxide dismutase(SOD)in the brain were determined; and striatum neuron apoptosis was detected by TUNEL method.
Results Levels of MDA in MnCl₂ group were higher than that in control group, and levels of MDA in GSH and LA group were lower than that in MnCl₂ group.Apoptosis rate of striatum neuron in MnCl₂ group was higher than that in control, GSH and LA group.
Conclusion Treatment with GSH and LA on rats could antagonize oxidative damage of brain and striatum neuron apoptosis induced by manganese.

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