Abstract: ObjectiveObserve the effect of temperature,p H and their interaction on Penicillium citreoviridin's(P.C.V.)toxin production in laboratory.MethodP.C.V.was cultured in Charles culture medium in different conditions according to the factorial experiment for two factors.CIT was extracted by dichloromethane and quantitated by HPLC method.ResultsP.C.V.produced CIT in all Charles culture medium.Quantities of CIT produced by P.C.V.were 60.30～336.10μg/g medium.P.C.V.produced more CIT in warm condition in acid Charles culture medium.ConclusionQuantities of CIT produced by P.C.V.were affected by temperature,p H and their interaction.