Abstract: ObjectiveThe vaccines produced according to the three techniques with the virus seeds R1203 of H5N1 avian influenza were used to immunize animals and to observe the effect.MethodsRats and rabbits were immunized with the different dose of the three vaccines of avian influenza(H5N1)by an intramuscular injection twice(interval 14 days).Blood was collected intravenously in the day of 14 and 28.The hemag glutination inhibition antibody and neutr alization antibody occurred in the serum of the animals after the first and second inoculation.ResultsThe antibody reaction in the rats was more sensitive than the rabbits.The rats and rabbits both could produce hemagg lutination inhibition antibody in 14th day with the titer of the antibody reaching at 1:40 while the titer of the neutralization antibody was very low and hardly detected.The second inoculation were significantly higher than the firstinoculation.The antibody reaction after inoculation of the split-vaccine in the rats and rabbits were significantly stronger than that inoculated with another two vaccines.The quantum-effect reaction was distinctin the rabbits.ConclusionThe production technique of split-vaccine was better than the other two.The neutralization antibody can accurately reflect the quality of vaccines.The efficiency test should be added in vaccines research and production with the method of immune rabbits.Itis sugg ested to immunize rabbits twice with 12μg HA with the titer of the sera-neutr alization antibody reaching at 1:40 or over in animal test before clinical trial.