Abstract: ObjectiveThe vaccines were produced by the designed techniques with the seeds(R1194 and R1203)of avian influenza viruses and checked entirely.A better one from the two seeds was selected to immunize animals for the next research.MethodsThe three purified techniques of the vaccines(the whole viruses、split-1 and split-2)were designed and the vaccines were produced with the two imported virus seeds.The purified condition of the vaccines was perfected.A better one from the two seeds was chosen to immunize animals.ResultsThe multiplication titers in the two viruses were higher at the inoculation titer of 10⁴ EID₅₀/ml(0.2ml/egg)incubation temperature of 34.5℃and incubation time of three days.The four items of endotoxin、egg-albumin、total albumin and total albumin/hemoglobin,which reflected the vaccine quality in the all produced vaccines,were better than the national standards.The form of the vaccines produced by the three techniques was totally different with electron microscopy.The albumen zone form with SDS-PA GE electrophoresis were similar in the whole virus vaccines and split-2 vaccine while the albumen zone of the split-1 vaccine could not be seen in 32 KD right-and-left.The vaccine output with the seed R1203 was much more than that with the seed R1194.ConclusionThe high quality vaccines can be produced by the three production techniques for purified vaccines.The vaccine output with R1203 is more than that with R1194.The vaccines produced by the three different techniques with the R1203 virus seeds can be used to immunize animals and to observe their antibody response.