砷暴露小鼠肝组织核酸损伤免疫组织化学观察

Observation on nucleic acid damage of liver in mice exposed to arsenic in low dose by immunohistochemistry

摘要: 目的为进一步研究砷对肝脏的毒性作用机制提供实验依据。方法昆明种小鼠40只,随机分为4组:1,2,4 mg/L三氧化二砷染毒组和生理盐水组,连续染毒60 d,取小鼠肝组织固定,用免疫组化方法观察肝脏组织细胞的8-Nitroguanine表达。结果染砷组小鼠肝细胞出现抗8-Nitroguanine免疫染色阳性反应,并随着砷暴露剂量的增加而呈现明显的剂量-反应关系。结论慢性低剂量砷暴露可诱发小鼠肝细胞8-Nitroguanine的高表达,提示8-硝基鸟嘌呤核苷(8-Nitroguanine)可以作为砷对肝细胞毒性作用的早期敏感生物标志物。

Abstract: ObjectiveTo provide evidence for exploring the mechanism of arsenic-induced hepatoxicity.MethodsForty mice were diveded into 4 groups,e.g.,one controls and 3 experimental groups(1 mg/L As₂O₃,2 mg/L As₂O₃ and 4 mg/L As₂O₃).As₂O₃ was administered for 60 days.Immunohistochemistry method was used to investigate 8-Nitroguanine expression in liver tissue of mice.ResultsExpression of 8-Nitroguanine was observed in the liver tissue of mice exposed to arsenic and there was relationship of dose-response in expression of 8-Nitroguanine with the increase arsenic dose.ConclusionArsenic induced high expression of 8-Nitroguanine in liver tissue of mice.8-Nitroguanine might be an earlier sensitive biomarker for arsenic toxicity to liver.