Abstract: Objective To research expression of sera TNF-αand IFN-γ/IL-4 in acute hepatic failure models induced by D-glactosamine and endotoxin.Methods Wistar rats with acute hepatic failure(AHF group,n=30)were injected with saline solution.Sera Alanine Transaminase(ALT)were determined and liver pathological morphology were observed at 3,6,12,24,48,72 h following intraperitoneal injection of D-glactosamine and endotoxin,5 rats per time point;simultaneously,sera TNF-αand IFN-γ/IL-4 were examined by ELISA.Results Infiltration of inflammatory cells and necrosis of hepatic cells were marked in AHF group,ALT increased significantly in AHF group than in N group(P<0.05)at all time points;Compared with N group,levels of sera tumor necrosis factor-α(TNF-α)in AHF group were obviously higher at 3 h(P<0.01)and 6 h(P<0.05),and interferon-γ(IFN-γ)were obviously higher at 3 h(P<0.01)and 6 h(P<0.01);interleukin-4(IL-4) weren't obviously higher at all time points(P>0.05).Conclusion TNF-α and IFN-γ play important roles in the AHF models at early phase;However,IL-4 may not contribute to the AHF rats induced by D-glactosamine and endotoxin.