Abstract: Objective To study new type drinking water of four kinds of ecological water,activate water,alkaline ion water and pure water effect on mice function of anti-fatigue and learning memory.Methods 120 Kunming mice were divided into four gr oups which drink this new type water.To breed intercourse male and female mouse after 3 experimental months and to get more filial generation mice that drink the same new type drinking water.After 20 experimental weeks,the female filial generation mice were used for caring on the heavy swimming experiment,and to measure the serumurea nitrogen content;the male filial generation mice were used for avoiding dark box experiment at the same time,so that it measure mice anti-fatigue and learning memory function respectively.Results The mice swimming time in activate water and alkaline ion water groups were longer than pure water group,and the mice serumureanitrogen content in alkaline ion water group was lower than pure water group.Although learning memory function did not show the obvious difference among groups.Conclusion A lkaline ion water has some anti-fatigue function for filial generation mice,but ithas no obvious influence on mice learning memory function from new type drinking water.