Abstract: Objective To investigate the efficacy of nano-silver bionic wound dressing.Methods Sprague Dawley rats (SD rats)were randomly divided into three groups:nano-silver dressing group(n=14),sulfadiazine silver(SD-Ag)group (n=14)and chitosan film group(n=14),and then deep partial-thickness wound involving 10～13%total body surface area(TBSA)were reproduced in the three groups.Blood samples on post-operation day 2,4,5,7,13 and 16 and liver,brain and kidney on post-operation day 45 were harvested for the determination of trace silver.Results The wound healing rate was higher in nano-silver dressing group than that in other two groups(P<0.01).The silver level in tissues and blood was lower than SD-Ag group(P<0.01).The blood silver content in nano-silver group returning to normal level was faster than that in SD-Ag group.Conclusion The nano-silver wound dressing can promote the healing of wound,and it can reduce the possibility of silver poisoning.Its good efficacy and safety shows a prosperous future in clinical setting.