Abstract:
Objective To investigate the antioxidant activities of taur ine on sarcoma 180(S
180)bearing mices.
Methods Sixty Kunming mices were divided randomly into five groups.Groups A,B,C were drank with 0.5%,1.0%,1.5% taurine respectively;group D was cyclophosphamide positive control;group E was the tumornegative control.Then mices were vaccinated with 0.2 ml S
180 cell suspension in leftaxillas.24 hours later,the positive control was injected by cyclo phosphamide 20 mg/(kg·bw).After 1 week,the content of maleic dialdehyde(MDA)and the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in plasma and liver were detected with test kit respectively.Erythrocytic haemolysis rate induced by H
2O
2 was studied.L ymphocyte were separated for determination of DNA damage by using SCGE.
Results Compared with negative control,the activity of SOD and GSH-Px in plasma and liver of taurine groups increased(
P<0.05 or
P<0.01)and the content of MDA in plasma decreased(
P<0.01)significantly.Erythrocytic haemolysis rate of 0.5% and 1.5% taurine groups were markedly lower than the negative control(
P<0.05).There was a decr easing tendency of the oxidative DNA damage on peripheral lymphocytes induced by 5,10 and 25 μmol/L H
2O
2.The DNA damage was obviously lower than the negative control in 1.0% and 1.5% taurine groups induced by 10 mol/L H
2O
2 and in 1.5% taurine group in duced by 25 μmol/L H
2O
2(
P<0.05 or
P<0.01).
Conclusion Taurine can improve antioxidative effect of S
180,bear ing mices,which may be associated with its effects of increasing antioxidase activity and reducing lipid peroxidation and protecting the membrane of erythrocytic and resisting oxidative DNA damage.