伤寒和甲型副伤寒沙门菌复合PCR检测

A multiplex PCR method to identify salmonella enterica serovars Typhi and Paratyphi A

摘要: 目的建立用于快速和特异检测伤寒沙门菌和甲型副伤寒沙门菌的复合PCR方法。方法根据rfbS、rfbEf、liC和vi基因序列设计4对PCR引物,对54株沙门菌和其他菌属的菌株进行复合PCR检测。结果采用所建立的复合PCR方法可分别检测到伤寒和甲型副伤寒沙门菌的3条和2条特异性条带带型,可与其他菌株带型准确分开。结论本研究建立的复合PCR方法可用于检测伤寒沙门菌和甲型副伤寒沙门菌。

Abstract: Objective To developa multiplex PCR method for identification of salmonella enterica serovars Typhi and Paratyphi A.Methods The PCR primers for O,H,and Viantigen genes,rfbE,rfbs,flic-a,and viaB,were designed and used for detecting 54 tested bacteria strains,including 47 Salmonella strains and 7 other bacterial strains.Results All the examined isolates of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.Conclusion The multiplex PCR can identify Salmonella enterica serovars Typhi and Paraty phi A with high specificity.