环境中条件致病菌对无机砷毒性降解作用

Study on arsenic resistance of the selected microorganisms in environment

摘要: 目的研究环境中几种常见的条件致病菌对无机砷的降解作用。方法各菌株接种在不同浓度含砷培养基中,观察其对砷的耐受性及砷对各菌株的生长影响;利用超声波细胞粉碎机震碎菌体后,用超低温捕获原子吸收分光光度仪检测砷含量,用荧光光谱检测器检测砷形态。结果实验所用各菌株均可耐受1 000 mg/L无机砷的浓度,养基中无机砷浓度<1 mg/L时对细菌的生长影响不大,无机砷浓度>1 mg/L时在短时间内可抑制细菌的生长,但随着培养时间的延长,细菌会耐受这种高砷环境。结论实验菌株对无机砷均具一定的降解作用。

Abstract: Objective To investigate arsenic resistance of the selected microorganisms in environment.Methods The selected microorganisms were cultivated at 37℃ in culture media that was put different concentration of sodium arsenite,and detected arsenic accumulation in cells by ASA.Results Coliform bacterium,Staphylococcus albus,Cerea bacillus,Bacillus aeruginosus and yeast fungus can grow when incubated in medium containing 1 000 mg/L arsenite.Conclusion The strains tested in the study have the ability of resistance to arsenic.