孕期壬基酚暴露对雄性仔鼠免疫功能影响
Effects of nonylphenol on immune function of F1 male rats after gestation exposure
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摘要: 目的 探讨孕期壬基酚暴露(NP)对雄性仔鼠免疫功能的影响.方法 母鼠孕14~19 d灌胃NP(0,20,40,80,200 mg/kg),仔一代(F1)雄性仔鼠于60 d龄剖杀取脾脏、胸腺、血清,测免疫功能相关各项指标.结果 NP80、200 mg/kg组脾脏、胸腺重量及脏器系数显著低于阴性对照组(P<0.05),且脾脏重量、脏器系数与染毒剂量呈负相关(r=-0.884);NP200 mg/kg组仔鼠血清γ-干扰素(IFN-γ)、白细胞介素6(IL-6)的水平与阴性对照组比较显著降低(P<0.05),且血清IFN-γ与染毒剂量呈负相关(r=-0.980);NP200 mg/kg组脾淋巴细胞的增殖能力较阴性对照组低(P<0.05),脾、胸腺内淋巴细胞显著减少,出现灶状坏死.结论 壬基酚可通过胎盘屏障,200 mg/kg对雄性仔鼠免疫功能有抑制作用.Abstract: Objective To investigate the adverse effects of nonylphenol(NP)on immune function of F1 male rats after gestation exposure.Methods Pregnant rats were exposed to N P at doses of 0,20,40,80 and 200 mg/kg body weights,respectively from pregnant days 14 to 19.Indices related to their immune system were observed while the F1 male rats were sacrificed at the age of 60th days.Results Compared with the control group,the absolute and relative weight of spleen and thymus were decreased dramatically in the 80 and 200 mg/kg N P treated groups.In the 200 mg/kg N P treated group,the proliferative responses of murine spleen lymphocytes cultured in vit ro were suppressed.Cytokine productions of interferongamma and interleukin-6 in serum were markedly lower than those in the control group.Conclusion N P can get across the placenta barrier.Exposure to 200 mg/kg/day N P can inhibit the immune function in F1 male offspring rats.
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