Abstract:
ObjectiveTo determine whether ag matine and nimodipine can prevent the toxic effects of manganese on glu-tamine synthetase,phosphate activated glutaminase,Na
+-K
+-ATPase and Ca
2+-ATPase in rat striatum.
Methods32 Wistar rats were divided into four groups by weight at randomization.The first group was the control group,the second group was given MnCl
2 only.The third and fourth groups were given agmatine and nimodipine respectively.The administration lasted 4 weeks.24 hours after the lastinjection,the activities of glutamine synthetase and phosphate activ ated glutaminase in striatum,the activities of Na
+-K
+-ATPase and Ca
2+-ATPase in pallium were determined.
ResultsCompared with the control group,the activities of glutamine synthetase (50.41±5.47)U/(min·g·pro),Na
+-K
+-ATPase (2.13±0.15)Lmol/(h·mg)and Ca
2+-ATPase (0.56±0.28)Lmol/(h·mg) in the second g roup decreased significantly(
P<0.01).The activity of phosphate activated glutaminase (72.24±9.38)Lmol/min·g·pro)increased significantly(
P<0.01).Compared with the second group,the activities of glutamine snthetase,Na
+-K
+-ATPase and Ca
2+-ATPase in the third and fourth groups increased significantly(
P<0.01).And the activity of phosphate activated glutaminase decreased significantly(
P<0.01).
ConclusionAgmatine and nimodipine have certain protective effects on the excitotoxicity induced by manganese in Wistar rats.