Abstract: Objective To investigate HIV coinfection with hepatitis C virus through different infectious routes in different regions in China.Methods 174 HIV-1-infected former plasma donors(FPD),89 HIV-1-infected injected drug users(IDU)and 194 HIV-1-infected heterosexuals were enrolled.Demology,infectious route,data of plasma donor,injected drug,homosexual,heterosexual were investigated and HCV-Ig G were measured.Results The total posotive rate of HCV-Ig G was 63.24%,and 95.40% in FPD,98.88% in IDU,18.04% in heterosexual,respectively.The risk factor of HIV co-infected with HCV were FPD(OR=89.84,95% CI=40.31～200.24)and IDU(OR=363.72,95% CI=48.90～∞).The infected rate of co-infection in Henan and Xinjiang province were 19.81%,25.00%,it was more higher than 2.94% in Liaoning province.Conclusion The total posotive rate of HCV-Ig G in HIV infection indivduals was higher than common population.The rate of HCV infected in heterosexual was lower than that of FPD and IDU.FPD and IDU were risk factor of HIV co-infection with HCV.The different region and sex parther resulted in difference of HCV co-infection.