Abstract: Objective To study the characters of growth,anti-thermo,anti-acid and anti-alkaline of different Enterobacter sakaz akii strains.Methods The growth character of 22 E.sakaz akii strains were monitored by microplate luminometer.And the survival of the strains under high temperature,acid or alkaline environment was detected by plating method.Results The correlation between E.sakazakii counts by conventional plating and by microplate luminometer was very good(R²=0.92).22 strains were treated at 75℃ for 2 min,and only 1 strain was survival.22 strains were all died immediately after treated on alkaline condition.Conclusion The microplate luminometer method in growth curves analysis of E.sakaz akii strains was rapid and convenient.The differences of anti-thermal,anti-acid and anti-alkaline characters of different strains were observed by the method.