Abstract: Objective To investigate in vitro effects of matrine on C.albicans biofilms.Methods XTT reduction assay was performed to determine the effect of matrine on C.albicans biofilms and its adherence.T he effect o f matrine on morphog enesis of C.albicans biofilms was obser ved by inverted microscopy,and cytotox ic assay was used to measure the adverse effects of matrine.Results SMIC50,SMI C80 of matrine against C.albicans biofilms was 500,1000 Lg/ml,respectively.M atrine at the concentration of 1000 Lg/ml could inhibit the adherence of C.albicans at 0,1,2,4 h.M atrine at the concentration of 100 Lg/ml could inhibit the adherence at 0,1 h,and 10 Lg/ml,at 0 h,respectively.1000 Lg/ml of matr ine could inhibit biofilm formation,and cause 14.05% of hemolysis.Conclusion M arine shows potential activ ity ag ainst C.albicans biofilm in the study.