黄芪对高糖环境下细胞膜流动性影响
Protective effects of Radix Astragali on cell membrane fluidity of ECV 304 cells exposed to high level of glucose
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摘要: 目的 探讨黄芪注射液(RA)对高糖环境下ECV304细胞膜流动性的影响,为RA在糖尿病治疗中的应用提供实验依据。方法 体外实验中利用葡萄糖建立高糖环境,体外培养ECV304细胞,将细胞随机分为正常组、高糖组、黄芪保护组(RA)组和甘露醇高渗对照组(简称甘露醇组)。加入处理因素24 h后,收集细胞,测定各组细胞的细胞膜流动性和各组细胞产生OH,O2-、和丙二醛(MDA)水平。结果 高糖组细胞产生MDA水平明显升高,显著高于正常组、RA组和甘露醇组细胞(P<0.01);RA组细胞产生MDA水平显著低于甘露醇组(P<0.01),与正常组细胞比较,差异无统计学意义。高糖组细胞抑制OH、O2-的能力显著低于正常组、RA组和甘露醇组细胞(P<0.01);RA组细胞抑制OH、O2-的能力显著高于甘露醇组细胞(P<0.01),与正常组细胞比较,差异无统计学意义。高糖组细胞超氧化物歧化酶(SOD)活性显著低于正常组、RA组和甘露醇组细胞(P<0.01);RA细胞SOD活性与正常组细胞比较,差异无统计学意义。RA组细胞的细胞膜流动性显著高于高糖组细胞(P<0.01),与正常组细胞比较,差异无统计学意义。结论 黄芪通过抗氧化作用,可保护高糖环境下ECV 304细胞的细胞膜流动性。Abstract: Objective To explore the effects of RadixAstragali(RA)on cellmembrane fluidity of ECV 304 cells exposed to high level of glucose through elfin mating free radicals.Methods ECV 304 cells were divided into four groups:nounal cell group,high level glucose group(the cells were exposed to 35m Mglucose),RA group(the cells were exposed to 35m.Mglucose and 500μg/L,RA)and 25mM mannitol group.After the cells were treated for 24 h,the cells from all groups were collected and the levels of MDA,OH、O2- and activeties of SOD as well as the cell membrane fluidity were deter mined.Results The levels of MDA,OH、O2-,in RA group was significantly lower than that of in high level glucose group;the activities of SOD was significantly higher The cellm embrane fluidity was significantly higher than that of in high level glucose group.Conclusion Radix Astragali shows protective effects on cell membrane fluidity of ECV 304 cells exposed to high level of glucose.
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