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王静, 糜漫天, 韦娜, 王斌. n-6/n-3 PUFA对乳腺癌大鼠ER及p53表达影响[J]. 中国公共卫生, 2009, 25(1): 48-50. DOI: 10.11847/zgggws2009-25-01-22
引用本文: 王静, 糜漫天, 韦娜, 王斌. n-6/n-3 PUFA对乳腺癌大鼠ER及p53表达影响[J]. 中国公共卫生, 2009, 25(1): 48-50. DOI: 10.11847/zgggws2009-25-01-22
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WANG Jing, MI Man-tian, WEI Na, . Effect of different ratios of n-6/n-3 PUFA on the expression of ER and p53 in MNU-induced breast cancer rats[J]. Chinese Journal of Public Health, 2009, 25(1): 48-50. DOI: 10.11847/zgggws2009-25-01-22
Citation: WANG Jing, MI Man-tian, WEI Na, . Effect of different ratios of n-6/n-3 PUFA on the expression of ER and p53 in MNU-induced breast cancer rats[J]. Chinese Journal of Public Health, 2009, 25(1): 48-50. DOI: 10.11847/zgggws2009-25-01-22
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n-6/n-3 PUFA对乳腺癌大鼠ER及p53表达影响

Effect of different ratios of n-6/n-3 PUFA on the expression of ER and p53 in MNU-induced breast cancer rats

    摘要
  • 摘要: 目的 探讨不同比例的n-6/n-3多不饱和脂肪酸(PUFA)对N-甲基亚硝基脲(MNU)诱导的乳腺癌大鼠乳腺组织中雌激素受体(ER)和p53表达的影响。方法 雌性SD大鼠随机分为n-6 PUFA、10:1 n-6/n-3、5:1n-6/n-3、1:1 n-6/n-3和正常对照5组,前4组以50mg/(kg·bw)MNU单次腹腔注射诱导乳腺肿瘤发生,正常对照组注射等体积无菌生理盐水。给药后立即分组喂养不同饲料,在8和18周时处死动物,RT-PCR和蛋白印迹(Western blot)技术检测各组大鼠乳腺组织ER和p53表达。结果 4组乳腺癌大鼠乳腺组织中ER和p53的表达均较正常对照组((0.73±0.11),(0.08±0.01))有所升高,其中n-6组最高((1.32±0.18),(1.43±0.56)),其余各组随n-6/n-3比值减小而下降,1:1 n-6/n-3组((0.95±0.12),(0.12±0.06))显著低于n-6组(P<0.05)。结论 不同比例n-6/n-3多不饱和脂肪酸对MNU诱导的乳腺癌大鼠乳腺组织ER和p53的表达具有不同影响,1:1 n-6/n-3膳食脂肪酸构成能有效抑制乳腺癌大鼠乳腺组织ER和p53表达的升高。

     

    Abstract: Objective To observe the effect of differen tratios of dietary n-6/n-3 poly unsaturated fatty acid on the expression of ER and P53 in the MNU-induced breast cancer rats.Methods Female SD rats were randomly divided into n-6 FUFA,10:1(n-6/n-3),5:1(n-6/n-3),1:1(n-6/n-3)and normal control groups.Except control group given physiological saline,all rats were given a singlipin jection of MNU50mg/(kg·bw)and switched to different diets containing fatty acids.After 8 weeks and 18 weeks,the expression of ER and p53 in the breast tissue of different groups were determ ined by RT-PCR and We stern blot.Results The expression of ER and p53 in breast cancer rats were higher than that of normal control(0.73±0.11,0.08±0.01)with the highest in n-6 group(1.32±0.18,1.43±0.56)and gradually decreased with the decreasing ratio of n-6/n-3.The expression of ER and p53 in 1:1 group(0.95±0.12,0.12±0.06)was significantly lower than that of in n-6 group.Conclusion Different dietary fatty acid compositions can diversely influence the expression of ER and p53 in MNU-induced breast cancer rats;1:1(n-6/n-3)can significantly inhibit the increasing of the expression of ER and p53 in breast cancerrats.

     

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