4a亚型猪戊型肝炎病毒对猕猴致病性

Study on pathogenicity of subtype 4a swine HEV in rhesus monkey

摘要: 目的研究4 a亚型猪戊型肝炎病毒(HEV)对猕猴的致病性。方法用1株从广西壮族自治区农村地区幼猪粪便中分离并已经基因测序确定为4 a亚型的猪HEV静脉注射感染猕猴,通过检测粪便HEV RNA、血中HEVRNA、血清丙氨酸氨基转移酶(ALT)水平、HEV抗体及肝组织分别进行病理学和电镜检查确定HEV致病性。结果猪HEV株实验感染猕猴后,出现粪便排毒、病毒血症、ALT升高及HEV抗体阳转,活检肝组织切片呈现典型的急性病毒性肝炎的病理变化,电镜下观察到肝细胞中散在有大量病毒样颗粒。结论广西4 a亚型猪HEV能够跨物种感染猕猴并引起戊型肝炎。

Abstract: Objective To investigate the pathogenicity of subtype 4a swine HEV in rhesus monkey.Methods Inoculate a rhesus monkey through intravenous injection with the subtype 4a swine hepatitis E vims (HEV)isolated from piglets fecal in Guangxi and identified by sequencing and evaluate its pathogenicity by detecting HEV RNA,semm ALT,anti-HEV and histopathological changes of liver biopsies.Results The infected monkeys had vims shedding,viraemia,elevated ALT and histopathological changes typical for acute vimal hepatitis.In addition,vims particles were observed under electron microscope.Conclusion Subtype 4a swine HEV could experin entally infect rhesus monkey and induce hepatitis E.