Abstract: ObjectiveTo investiga te the effects of Yiqijiedu formula on cell proliferation of nasal and nasopharyngeal epithelia and the expressivelevels of TRAF2 and p16 in TgN(p53mt-LMP1)/HT mice.MethodsThe cell proliferative characteristics of nasalm uco sa and na sopharyngeal epithelia was evaluated by H-E staining procedures and the expressive levels of TRAF2 and p16 genes were assayed by immunohistochemical methods.At the same time,the genes expressive levels were also identified by western blot. ResultsCompared with tho se of TgN(p53mt-LMP1)/HT mice of normal saline (NS)group(TIC group),hyperp lasia of nasal and/or nasopharyngeal epithelia was not obvious in TgN(p53mt-LMP1)/HT cancerous lesion inducing mice with Yiqijiedu fromula(TMI group)and the occurringrate of precancerous lesions in nasal and/or nasopharyngeal epithelia was reduced significantly(P<0.01).The TRAF 2 expressivelevels(129.45±9.53, 160.96±11.04 of nasal cavity epithelia of TIC group,and TIM group;126.36±12.69,160.88±6.84 in naso phary geal epithelia)we re decrea sed(P<0.01)and p16 expressivelevels(157.21±10.44,131.72±10.06 in nasal epithelia of TIC and TIM group;155.41±11.77,132.63±10.22 in na sopharyngeal epithelia of TIC and TIM group)were increased markedly(P<0.01).TRAF2 and p16 expressive levels were similar to those of C57 BL/6J wild type mice(TRAF2 gene: 165.07±10.58 in nasal epithelia,164.39±7.88 in naso phary geal epithelia;p16 gene:129.62±12.16 in nasal epithelia and 129.12±11.67 in nasopharygeal epithelia)with no significant differece.ConclusionThe effects of N,N'-dinitro sopipera zine(DNP)on nasal/naso phary ngeal epithelial abnormal proliferation of TgN(p53mt-LM P)/HT mice can be inh ibited by Yiqijiedu formula herbal medicine.The effects of Yiqijiedu fromula is closely related with TRAF2 gene down regulation expression and p16 gene expression upregulation in nasal/naso pharyngeal epithelia of TgN(p53mt-LMP1)/Ht mice.