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裴凌鹏. 叶黄素对脂多糖致小鼠急性肺损伤保护作用[J]. 中国公共卫生, 2009, 25(4): 461-463. DOI: 10.11847/zgggws2009-25-04-38
引用本文: 裴凌鹏. 叶黄素对脂多糖致小鼠急性肺损伤保护作用[J]. 中国公共卫生, 2009, 25(4): 461-463. DOI: 10.11847/zgggws2009-25-04-38
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PEI Ling-peng. Study on protective effects of lutein on acute lung injury induced by lipopolysaccharide in mice[J]. Chinese Journal of Public Health, 2009, 25(4): 461-463. DOI: 10.11847/zgggws2009-25-04-38
Citation: PEI Ling-peng. Study on protective effects of lutein on acute lung injury induced by lipopolysaccharide in mice[J]. Chinese Journal of Public Health, 2009, 25(4): 461-463. DOI: 10.11847/zgggws2009-25-04-38
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叶黄素对脂多糖致小鼠急性肺损伤保护作用

Study on protective effects of lutein on acute lung injury induced by lipopolysaccharide in mice

    摘要
  • 摘要: 目的研究叶黄素对脂多糖(LPS)所致小鼠急性肺损伤(ALI)的保护作用。方法雄性昆明种小鼠60只,随机分为正常对照组、急性肺损伤模型组、地塞米松阳性对照组(5mg/kg)以及叶黄素低、中、高剂量组(10,15,20mg/kg)共6组。不同剂量叶黄素给大鼠连续灌胃30d后,腹腔注射脂多糖6.0mg/kg建立ALI模型。在注射后6h,收集腹主动脉血并进行左侧支气管肺泡原位灌洗以收集灌洗液,测定血中淋巴细胞亚群CD3+、CD4+、CD8+、肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)及丙二醛(MDA)及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性;测定各组的肺湿重/干重比、肺组织中性粒细胞髓过氧化物酶(MPO)含量及肺组织匀浆TNF-α、白细胞介素10(IL-10)含量。结果叶黄素各剂量组的血TNF-α含量由低到高依次为(390.10±81.50),(374.20±80.09),(340.18±84.39)ng/L,低于模型组(475.29±93.12)ng/L;IL-8含量由低到高依次为(111.13±16.30),(107.33±15.39),(103.39±14.36)ng/L,低于模型组(124.56±20.21)ng/L;MPO含量由低到高依次为(5.12±1.02),(5.03±0.80),(4.48±0.73)U/g低于模型组(6.02±1.06)U/g;MDA含量由低到高依次为:(9.20±0.62),(8.29±1.30),(7.10±1.21)nmol/(mgpro),低于模型组(11.28±2.14)nmol/(mgpro);肺组织湿重/干重比亦低于对照组,差异均有统计学意义(P<0.001),表明叶黄素各剂量组均可降低LPS诱发的过氧化反应。同时叶黄素各剂量组的IL-10含量由低到高依次为(71.23±22.39),(78.28±21.27),(85.18±28.15)ng/L,高于模型组(60.13±20.28)ng/L;血SOD含量由低到高依次为(114.30±41.50),(130.53±40.23),(149.19±41.77)U/(mgpro),高于模型组(74.52±24.40)U/(mgpro);GSH-Px活性由低到高依次为(77.70±12.15),(85.20±12.03),(90.47±13.12)U/(mgpro),高于模型组(62.24±10.13)U/(mgpro);并改善血中淋巴细胞亚群分布;差异均有统计学意义(P<0.001),表明叶黄素各剂量组均可拮抗LPS引起的抗氧化机制的损伤。结论叶黄素对LPS所致急性肺损伤具有预防性保护作用。

     

    Abstract: ObjectiveTo study the protective effects of lutein against acute lung injury induced by lipopolysaccharide and its possible mechanism in mice.MethodsRandomly distributed 60 male Kunming mices into control group,lipopolysaccharide-induced acute lung injury model group(ALI),dexamethasone(DXM)group(5 mg/kg)and low,middle,high dose groups of lutein(10,15,20 mg/kg).After 30d with different dosage of lutein,control group was given physiological saline,ALI model group,lutein administrated groups and DXM group were injected with lipopolysaccharide(LPS)(6.0 mg/kg)to induce ALI.Six hours after the injection,abdominal aorta blood was collected for measuring of lymphocyte subpopulations(CD3+,CD4+,CD8+),tumour factor-α(TNF-α),leckocyte interpose-8(IL-8),malondialdehyde(MDA)content,superoxide dismutase(SOD),glutathione perioxidase(GSH-Px)activities.And lung wet weight/dry weight ratio,neutrophil MPO activity of lung TNF-α,leckocyte interpose-10(IL-10)content of lung were measured.ResultsTreatment with different dosage of lutein could significantly decrease serum TNF-α(model group:475.29±93.12;lutein groups from low dose to high dose:390.10±81.50,374.20±80.09,340.18±84.39);IL-8(model group:124.56±20.21;lutein groups from low dose to high dose:111.13±16.30,107.33±15.39,103.39±14.36);MPO(model group:6.02±1.06;lutein groups from low dose to high dose:5.12±1.02,5.03±0.80,4.48±0.73);MDA content(model group:11.28±2.14;lutein groups from low dose to high dose:9.20±0.62,8.29±1.30,7.10±1.21).There was a reduction of IL-10 in lung(model group:60.13±20.28;lutein groups from low dose to high dose:71.23±22.39,78.28±21.27,85.18±28.15).The concentrations of SOD were(model group:74.52±24.40;lutein groups from low dose to high dose:114.30±41.50,130.53±40.23,149.19±41.77).GSH-Px activities were(model group:62.24±10.13;lutein groups from low dose to high dose:77.70±12.15,85.20±12.03,90.47±13.12).The lymphocyte subpopulations of the blood was improved.ConclusionLutein showed protective effects on the acute lung injury induce by LPS in mice.

     

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