Abstract: ObjectiveTo study the inhibitory effect of ethanol extract of Liuyueqing(EELYQ)on duck hepatitis B surface-antigen(DHBsAg)and hepatitis B e-antigen(DHBeAg).MethodsOne day old Guangxi brown spotted ducks in fected with DHBV were used as the hepatitis B virus infection animal model.DHBsAg and hepatitis B positive ducks were detected by PCRat 13 day after stopping the infection of DHBV,and were randomly divided into five groups:the high,middle and low dose EELYQ groups,model group,positive control group.Each group had 10 ducks.EELYQ was given.i g.for 14 days,and the contents of DHBsAg and DHBeAg in serum were measured by ELISA.ResultsThe serum DHBsAg and DHBeAg contents were decreased significantly by the treatment of EELYQ.The high and middle dose of EELYQ could significantly suppress DHBsAg and DHBeAg expressions in vivo(P<0.01).DHBsAg and DHBeAg contents in serum of high dose group did not return significantly 3 days after stopping treatment.The DHBsAg inhibition rate was 51.85% and 59.04%,respectively at 14 days treatment and 3 days after stopping the treatment in high EELYQ dose group and for DHBeAg,the inhibition rate was 34.27% and 39.96%,respectively.The inhibitory effect of EELYQ was dose and time dependent.ConclusionEELYQ can significantly inhibit the expressions of DHBsAg and DHBeAg.