Abstract: ObjectiveTo learn the isolation rate and the profile of drug resistance of Gram-negative bacilli in ICU ward in order to provide the basis for rational administration of antibacterial and infection prevention.MethodsIdentification of bacteria was performed by ATB-expression system and the drug sensitivity test was conducted with Kirby-Bauer method.The detection rate and drug resistant rate were analyzed with WHONET 5.4 software.ResultsTotally 677 strains of Gram-negative bacilli were isolated from various clinical specimens in ICU ward from 2005 to 2007.The predominant pathogens were Pseudomonas aerations(3910%),Acinetobacter Baumann(17.0%),Klebsiella pneumonia(16.8%), Stenotrophomonas Maltophilia(11.4%),Escherichia coli(4.3%).Among them,56.3% Klebsiella pneumonia strains produced ESBLs,24.1% of Escherichia coli produced ESBLs.504(74.5%)strains of non-fermented were isolated.The antibiotic resiance of non-fermented strains to Caffeine,Meropenem,Piperacilli, Tazobacyam and Ceftazidmie was 49.3%,50.4%, 51.8% and 57.9%,respectively.The resistant rate of fermenter straines to Reopened,Piperacillin/Tazobac tam,Amice,Caffeine and Ceftazidime was 0%,34.5%,45.3%,54.3% and 61.3%,respectively.ConclusionGram-negative bacilli are main pathogen in ICU ward,non-fermented are predominant patho gen,and the detection rate of Pseudomnas are the highest.