氟对大鼠自由基、氧化应激及超微结构影响
Effect of fluoride on free radical,oxidative stress and ultrastructure of liver in rats
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摘要: 目的 研究不同浓度氟对大鼠肝脏自由基和氧化应激及超微结构的影响.方法 在饮水中加入不同剂量的氟化钠喂饲大鼠1个月后,测定血清氟含量,肝组织中的一氧化氮(NO)、丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性,并观察大鼠肝脏超微结构变化.结果 随染氟剂量的增加,1个月后各剂量组大鼠血氟浓度相应增加,存在剂量-效应关系(R=0.884,P<0.01),但各剂量组间血氟浓度差异无统计学意义;NO含量与对照组比较差异无统计学意义(P>0.05),但存在剂量-效应关系(R=0.361,P<0.05);各剂量组大鼠MDA含量升高,与对照组比较差异有统计学意义(P<0.01),存在剂量-效应关系(R=0.797,P<0.01);各剂量组大鼠GSH-Px活性下降,与对照组比较差异有统计学意义(P<0.01),存在剂量-效应关系(R=-0.912,P<0.01),特别是高氟剂量组MDA的含量最高,而GSH-Px活性最低;中、高剂量组大鼠肝细胞超微结构可见线粒体肿胀,有较多脂肪变性.结论 氟中毒可导致大鼠肝脏处于氧化应激状态、肝细胞超微结构改变.Abstract: Objective To study the effect of different dosages of fluoride on free radicals,oxidative stress and ultrastructure of liver in rats.Methods Drinking water with various concentrations of sodium fluoride was given to rats for one month.The contents of fluoride,nitnic oxide(NO),maleic dialdehyde(MDA)and activities of glutathion peroxidase(GSH-Px)in the liver of rats were measured.Results The serum fluoride increased in dose-effect manner with the fluoride exposure(R=0.884,P<0.01).There was no significant difference in NO between exposure and control groups(P>0.05).The liver MDA of experimental groups increased with a dose-effect trend compared with the contral group(R=0.797,P<0.01),and the GSH-Px decreased with the fluoride exposure(P<0.01;R=-0.912,P<0.01).The swollen mitochondrion and degeration of fatty cells were observed in midium and high fluoride exposure groups.Conclusion Fluoride exposure may induce oxidatives tress and ultras tructure changes of the liver in rats.
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