Abstract: Objective To study the an tibiotic resistance of Vibrio cholerae in Hainan province,and to provide the references for clinical treatment and prevention and control of cholera.Methods Eighty one samples from outbreak of cholera epidemic were collected in Hainan province in 2008.The antibiotic susceptibility of the 81 strains to 18 kinds of antibacterial was determined by improved KB method recommended by WHO.Results All the 81 strains were susceptible to norfloxa cin,cefotaxmie and cephalothin.The rate of resistance to streptomyc in,sulphame thox azole/trmiethoprmi,sulphafurazole and polymyx in B were 100%.The rate of resistance to tetracy cline was 92.59%(75/81).Conclusion Norfloxac in,ce fotax mie and cephalothin could be the choice of clinical treatment for case and carrier of Vibrio cholerae in Hainan province.But streptomy cin,sulpham ethoxazole/trimethoprmi,sulphafurazole,polymy xin B,and tetracychine could not be used.