Abstract: ObjectiveTo collect information on psychoactive sbustance use among universtity and middle school students in Guanzhou, and to provide basis for intervention measures.MethodsA self-admroistered questionnaire survey was conducted among 4594 students from 10 universities and 8 middle schools in Guanzhou.ResultsAmong 4594 students, 26.1% ever tried cigarete smoking(one or more puffs);the prevalence of current cigarette use was 6.2%.62.4% of the students tried alchol drinking; 14.0% had accident injury after drinking; 4.3% had compound cough syrup use experience.The precentage of the students who reported illegal drug use was 1.2%.The prevalence of psychoactive substance use was higher among the male than in the female.Students who had characteristics such as loneliness, out of harmony with other person, bad performante record, and bad family environment had higher prevalence of psychoactiove substance use than other students.ConclusionThe prevalence of psychoactive substance use among university and middle school students should not be ignored.It is necessary to implement comprehensive prevetion in school, family and society.