Abstract: Objective To research the effect of Rana japonica oil on immunity factors and learning ability of the mice under the condition of hypergravity and microwave radiation.Methods The mice under hypergravity and microwave radiation were treated with Rana japonica oil.The micepsse ruminte rleuk in-10(IL-10),interferon-gamma(IFN-C),and tumor necrosis-alpha(TNF-A)were determined and the learning ability was observed with step-through and step-down test.Results IL-10,IFN-C,and TN F-A of Rana japonica oil group showed significant difference from that of hypergravity and microwave group(P<0.05);the latency period and the wrong number in step-through test and step-down test of treated mice were obvioursly different from that of before training(P<0.05).Conclusion The Rana japonica oil may streng then mimunity fuction of mice under hypergravtity and microwave radiation.