Abstract: Objective To determine the antibiotic resistance of Helicobacter pylori(H.pylori)strains isolated from the patients with chronic gastritis or peptic ulcer in Suzhou area,and to provide information for control of H.pylori infection.Methods H.pylori was isolated from gastric mucosal biopsy specimens obtained from 506 patients with positive rapid urease test(RUT)result.The susceptibility to an tibiotic was determined by Kirby-Baner test.Results The positive rate of isolated H.pylori strains was 76.1% among 506 patients.Overall the resistance rates for metron idazole and clarithromy cin were 75.5% and 19.4%,respectively(P<0.001,P<0.05).But H.pylori remained highly susceptible to amoxicillin,furazolidone,levofloxacin,cefuroxmie,and moxifloxacin with resistant rates of 3.6%,2.2%,7.2%,0.0% and 5.8%,respectively.The positive rate of H.pylori cultivation increased with the increase of the positive rate of RUT.Conclusion The first-line antibiotic treamtent for H.pylori infection in Suzhou area should be amoxicillin,furazo lidone,levofloxacin,cefuroxmieor moxifloxacin,and excky demetron idazole.