亚硫酸钠对人胚肾293细胞APP蛋白表达影响

白剑英; 王明衡; 王慧宇; 梁瑞峰

doi:10.11847/zgggws2010-26-02-43

亚硫酸钠对人胚肾293细胞APP蛋白表达影响

Effect of sodium sulfite on expression of β-amyloid precursor protein(APP) in human embryonic kidney 293 cells

摘要

摘要: 目的研究食品添加剂亚硫酸钠(Na₂SO₃)对β-淀粉样物质(Aβ)前体蛋白(APP)表达的影响。方法用反转录-聚合酶链反应(RT-PCR)检测人胚肾293(HEK293)中APP基因的mRNA表达水平,用蛋白印迹法(West-ern-blotting)检测HEK293中APP蛋白水平。结果RT-PCR检测显示,HEK293接触0.039~10 mmol/L Na₂SO₃24 h后细胞内APP基因的3个亚型APP695、APP751、APP770的mRNA水平均未见明显改变。蛋白印迹分析发现,10mmol/L Na₂SO₃可明显降低细胞内APP蛋白水平,为阴性对照组的0.908倍;而较低剂量组0.039~2.5 mmol/LNa₂SO₃却可以明显增加细胞内APP蛋白的水平,分别达到阴性对照组的1.596,1.630,1.556和1.446倍。结论亚硫酸钠在一定剂量范围内影响HEK293细胞内APP蛋白水平,可能对老年性痴呆的发生有一定影响,但是细胞内APP蛋白水平的增加不是由该基因转录水平增加所致。

Abstract: ObjectiveTo investigate the β-amyloid precursor prote in(APP)gene changes induced by sodium sulfite.MethodsRT-PCR was used to study mRNA changes of APP and Western-blotting was used to detect the prote in level of APP in human embry onic kidney cell line 293(HEK 293)treated with sodium sulfite.ResultsRT-PCR results showed that treatment of 0.039-10 mmol/L Na₂SO₃ could not change the mRNA level of APP;Western-blotting results showed that 10 mmol/L Na₂SO₃ could decrease the protein level of APP in the cells significantly.On the other hand,0.039-2.5 mmol/L Na₂SO₃ could increase the prote in level of APP inside cells.Semi-quantity analysis showed that APP prote in levels were 1.596,1.630,1.556,and 1.446 times,respectively,when compared to that of the control.ConclusionNa₂SO₃ can cause significant increase of APP prote in level in HEK 293 and itmaybe related to Alzheimers' disease.But the change is not due to the change of mRNA of APP.

HTML全文

参考文献(12)

施引文献

资源附件(0)