中药乙醇提取物对肝癌细胞中乙肝抗原抑制作用
Inhibitory effects of 30 alcoholic extracts of Chinese medicine on HBsAg and HBeAg excreted by 2215 cell
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摘要: 目的 观察中药乙醇提取物在乙型肝炎病毒(HBV)DNA转染的人肝癌细胞系2215细胞培养中,对2215细胞HBsAg、HBeAg分泌的影响。方法 药物对细胞的毒性实验,采用2215细胞为模型,加药后显微镜观察细胞病变。在无毒浓度下,分别检测30味中药提取物对细胞培养上清液中乙型肝炎表面抗原(HBsAg)和乙型肝炎e抗原(HBeAg)的抑制作用。结果 通过计算抑制百分率和治疗指数,其中18味为抑制乙肝表面抗原HBsAg有效低毒药物,其中使君子表面抗原抑制率高达89.7%,治疗指数为22.8;女贞子表面抗原抑制率高达87.9%,治疗指数为13.8。6味为抑制乙肝e抗原HBeAg有效低毒药物,其中石榴皮对e抗原抑制率73.6%,地耳草对e抗原抑制率72.6%。结论 本实验方法作为快速体外筛选模型简便易行,筛选的有效药物如使君子、石榴皮等可进行深入的抗乙肝病毒的体内实验研究。Abstract: Objective To observe the effects of 30 alcoholic extracts of Chinese m edicine on HBsAg and HBeAg excreted by 2215 cell-hepatom a cells transfected by HBV-DNA.Methods The cytotoxicity tests were carried out with 2215 cell tredted with thirty different alcoholic extracts of Chinese medicine with cytopathic effect (CPE) observation.The inhibitory effects of 30 alcoholic extracts of Chinese medicine on HBsAg and HBeAg were observed at atoxic concentrations.Results Eighteen extracts of Chinese medicine showed obvious inhibitory effect on HBsAg and six extracts of Chinese medicine showed obvious inhibitory effect on HBeAg by the inhibitory rate and the therapeutic index.The inhibition rate of Ramuli euonymi and Ligustrum lucidum on HBsAg were 89.7% and 87.9%,with the therapeutic indexs of 22.8 and 13.8.The inhibition rate of Granatum and Diercao on HBeAg were 73.6% and 72.6%.Conclusion The method to screen antihepatitis vims B drugs in vitro is convenient and feasible.The effective medicine such as Ramuli euonymi and Granatum can be taken into further anti-HRV studies.
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