NF-κB对小鼠冠状病毒性肝炎致病作用
Expression of NF-κB and ThI/Th2 in mice with mouse hepatitis virus infection
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摘要: 目的 探讨肝脏核因子-κB(NF-κB)和血清辅助性T淋巴细胞(Th1/Th2)细胞因子方面对小鼠冠状病毒性肝炎的致病机制.方法 采用4周龄雄性BALB/c小鼠,以鼠肝炎冠状病毒(MHV-A59)和大肠埃希菌作为生物致病因子,建立MHV-A59鼠肝炎模型和大肠埃希菌鼠肝炎对照模型,以正常健康小鼠为空白对照,采用免疫组化法检测小鼠肝脏NF-κB表达吸光度(A)值,ELISA法检测小鼠血清白介素-4(IL-4)、干扰素-γ(IFN-γ)水平.结果 冠状病毒模型组、大肠埃希菌对照组小鼠肝脏NF-κB表达分别为A=0.305,A=0.245;外周血Th1/Th2(IFN-γ/IL-4)比值分别为(2.17±0.16),(2.09±0.06)pg/mL,均比正常组有明显升高,差异有统计学意义(P<0.05或P<0.01),但冠状病毒模型组升高更明显.结论 肝脏NF-κB表达增强及血清Th1/Th2细胞因子失衡可能是小鼠冠状病毒性肝炎的致病机制之一.Abstract: Objective To study expressions of NFB and Thelper 1(Th1)/The lper-(Th2)in mice with mouse hepatitis virus(MHV)infection.Methods Four weeks old BALB/c male mice were divided into three groups randomly:control group,MHV model group,and Bacillus coli group.The expression of NFB in mouse livertissue was analyzed with mimunoh istochemistry.The level of interleuk in-4(IL-4)and interferon gamma(IFN-γ)in mouse serum were detected with ELISA.Results The expressions of NFB and Th1/Th2(IFN-γ/IL-4)of MHV group and Bacillus coli group increased obviously compared to that of control group(P<0.05 or P<0.01).Conclusion The increased expression of NF-кB in mouse liver and the disequilibrium of Th1/Th2 in serumare one of pathogenic mechanisms of mouse hepatitis.
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