Abstract: Objective To investigate the am picillin and tetracyc line-resistant strains and resistant genes of lactic acid bacteria isolated from yogurt.Methods Lactic acid bacteria in yogurt were isolated and identified.Resistance to ampicillin or tetracyc line of the strains were investigated withs lipsuscep tibility test.Two pairs of primers were designed and am picillin and tetracyc line resistance gene were am plified.Results Sevenlactic acid isolates we reidentified from 4 kinds of yogurt.Anumber of isolates were resistant to ampicillin or tetracyc line.Five of the 7 lactic acid isolate contained ampic illin-resistant gene,and no isolate contained the tetracyc line-resistant gene.Conclusion A number of lactic acid bacteria were resistant to antibiotics and could act as in termediate host of antibiotic resistant genes.