Abstract: Objective To investigate congenetic genotype of methicillin resistant Staphylococcusau reaus(M RSA)isolated from different sections in a hospital for the control of nosocomial infection.Methods Fiftys trains of MRSA isolated from nosocomial infection inpatients were detected for genotyping with automated Diversilab and repetitive-sequence-based polyme rasechain reaction(REP-PCR).Results The MRSA strains were classified into 19 different genotypes and the major type was type A(20 strains).The strains of type A were isolated mainly from the patients in 11 words such as intensive careunit,departments of rehabilitation,respiratory,neurosurgical,and cardiac surgery.The number of type B,D,J,H,and strain L was 7,3,3,2,and 2,respectively,with only one strain of other type.Conclusion There is a prevalence of the type As train of MRSA and the strain may be disseminated in cloning ways.