Abstract: Objective To establish an acute hyperlipidemia model with yolk and TritionW R 1339 in mice.Methods A cuteanmial models of hyperlipidemia were established in mice with 75% yolk peritoneal in jection(mode lA) and 3% Trition WR 1339 solution caudal vein injection(model B),respective ly.Inadditio n,the effects of smivastatin treamtent on the two acute hyperlipidemia models were investigated.Results After the yolk peritoneal injection,serumtrig lyceride(TG),total chole sterol(TC),low density lipo prote in cholesterol(LDLC),and high density lipoprote in cholesterol(HDLC) of model A were 14.02,7.33,2.98,and 1.03 mmol/L,respectively,which were significantly higher than those of control group A(P < 0.01).After 3% Triton WR 1339 solution cauda lvein in jection,serum TG,TC,LDLC,and HDLC of model B were 16.87,8.71,3.83,and 1.27 mmol/L,respectively,which were significantly higher than those of control group B(P < 0.01).Smivastatin treatment to the mice in the two models significantly decreased serum TG,TC,LDLC,and HDLC.Conclusion The application of 75% yolk peritoneal in jection and 3% Triton WR 1339 solution caudalve in injection can induce acute hyperlip idemia in mice.