Abstract: Objective To observe the effects of Jinku ishenqiwan and micronutrients on testosterone level and antioxidation in aging mice induced by Dga lactose.Methods The aging mice were divided into blank control,model control and four treamtent groups.The treamtent groups were treated with different dose of Jingkuishenqwi an or/and medicaments,respectively,and the control group with distilled water for 6 weeks.The serumtesto sterone,the activity of glutathion peroxi dase(GSHPx) and superox ide dismutase(SOD) in blood,lung,liver,brain and heartwere tested.Results The contents of testosterone for model control and four treatment groups were 1.734±0.102,1.826±0.069,1842±0 082,1.883±1.080,and 2.005±0.120 ng/m L,and the activities of GSHPx were 381.61±24.63,448.67±20.52,480.76±16.87,489.71±19.39,and 511.02±21.16 U/m l,respectively; the contents of SOD in blood were 13347.86±1147.86,16603.16±727.64,18378.42±908.91,18787.70±1322.91,and 19878.67±1148.77 U/g Hb for the mice in the model control group and four treamtent groups.There were obvious differences in the indexes between blank control group and model control group and four treamtent groups(P < 0.05 for all).The contents of GSH Px and SOD in lung,liver,bra in and heart of treamtent groups were increased significantly compared to that of the blank control group(P < 005).Conclusion Jingku ishenqiwan increasesse rumtesto sterone,the activity of GSH Px and SOD in the blood and tissues significantly,and shows a better effect when combined with micronutrient.