Abstract: ObjectiveTo explore the influence of multiple risks on the generation and development of atherosclerosis.MethodsMice were randomly divided into 6 groups:normal(group A),high fat diet(group B),Chlamydia pneumoniae (CPn)(group C),high fat diet+CPn(group D),CPn+methionine(group F),high fat diet+CPn+methionine(group E).All the mice were fed with specific diet or inoculated with CPn.The blood of the mice was collected to detect the level of blood fat,and thoracic aorta was taken to observe ultra-structrue changes.ResultsCompared with group A,the level of total cholesterol(TC),tryglyceride(TG),and atherogenic index of plasma(AIP)in other groups was obviouslv higher (P<0.01 for all).And the endothelial cells and smooth muscle cells in all groups except group A were damaged in some extent.Compared with group B,the level of TC,low density lipoprotein-cholesterol(LDL-C),and oxidized low density lipoprotein(ox-LDL)were higher(P<0.05 or P<0.01),and the level of TC,ox-LDL in grouo F were higher significantly (P<0.05 or P<0.01).The level of LDL-C in group C was obviously higher than that of in group A(P<0.05).ConclusionCPn infection promotes the development of dyslipidosis and aggravates dyslipidosis induced by high fat diet.CPn and methionine can promote genesis and development of atherosclerosis in mice.