Abstract:
ObjectiveTo observe the dynamics of intestinal intraepithelial lymphocyte subsets in mice intranasaly immunized with soluble tachyzoites antigen(STAg)in association with propolis and gamma interferon(IFN-γ).
MethodsNinety-six female BALB/c mice were randomly divided into immunized and control group and immunized with 2 doses of STAg plus propolis and IFN-γ against
Toxoplasma gondii and phosphate buffer,respectively,at an interval of 2 weeks.The mice were killed 1,2,3,4,6,8,10,and 12 weeks after the 2nd dose,respectively,and intestinal intraepithelial lymphocytes (IEL)were isolated and counted.Percentage of CD4
+ and CD8
+ T cells was determined by immunocytochemistry.
ResultsThe number of IEL of the mice in immunized group reached a peak value at week 2(2.27×10
5)and was significantly higher than that of in the control group during weeks 1-4(
F=8.42,
P<0.01).The numbers of CD4
+ and CD8
+T cells were both higher than those of the control.CD8
+ T cells was significantly higher at weeks 1,2,3,4,and 6 than those in the control group (
F=6.38,
P<0.05),while CD4
+ T cells in immunized group increased significantly at week 2 and 3(
F=5.64,
P<0.05). The ratio of CD4
+/CD8
+ T cells was higher at week 1 and 2(
F=5.74,
P<0.05).
ConclusionThe immunization with STAg in association with propolis and IFN-γ by intranasal drip can effectively induce immune responses of IEL.