Abstract: Objective To evaluate the effects of comprehensive intervention measures mainly consisting of health education class on health behavior among primary and middle school students and to provide reference for health promotion in school.Methods In Yiyuan county and Shouguang city,case-control study was used to survey 600 students in intervention group and 399 students in non-intervention group on health behavior.Results There was no significant difference in total awareness rate of health knowledge between intervention group(52.3%) and non-intervention group(54.4%) before the intervention(P > 0.05).After the intervention,the total awareness rate of health knowledge of the intervention group(70.9%) was significantly higher than that of the non-intervention one(54.4%).There was no significant difference in health-related attitude and behavior between the two groups before the intervention(P > 0.05).After the intervention,the health-related attitude and behavior in intervention group were significantly improved except “smoking is not the mature sign”(P < 0.05).After the intervention,the rate of students actively propagandizing health knowledge in the intervention group(90.0%) was significantly higher than the rate in non-intervention group(82.7%).Conclusion The level of health knowledge was improved and health-related belief and behavior were promoted by comprehensive intervention measures among primary and middle school students.